Evaluation of sample work-up methods and internal standards for the determination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in brain by HPLC with electrochemical detection

A high-performance liquid chromatographic method with electrochemical detection is described for the determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain. Results of studies on sample work-up methods and on the use of various internal standards are reported. T...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1988, Vol.6 (6), p.903-909
Hauptverfasser:	Duchateau, A., Martens, H., Teuchy, H., Schoups, A., Claeys, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	5-hydroxyindoleacetic acid 5-Hydroxytryptamine electrochemical detection reversed-phase high-performance liquid chromatography
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Beschreibung
Zusammenfassung:	A high-performance liquid chromatographic method with electrochemical detection is described for the determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain. Results of studies on sample work-up methods and on the use of various internal standards are reported. The reproducibility of determination of 5-HT and 5-HIAA in two regions of rabbit brain has been evaluated.
ISSN:	0731-7085 1873-264X
DOI:	10.1016/0731-7085(88)80108-0