Conventional and laser induced fluorescence detection of glucuronic acid conjugates after derivatization and liquid chromatographic separation

Pre-chromatographic derivatisation of the carboxylic acid function of glucuronic acid conjugates is a suitable method for the selective and ultra-sensitive analysis of these compounds in urine and plasma samples. This goal is achieved by applying an indirect derivatisation procedure and laser induce...

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Veröffentlicht in:Journal of pharmaceutical and biomedical analysis 1988, Vol.6 (6), p.687-695
Hauptverfasser: Lingeman, H., Tjaden, U.R., Van Den Beld, C.M.B., Van Der Greef, J.
Format: Artikel
Sprache:eng
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Zusammenfassung:Pre-chromatographic derivatisation of the carboxylic acid function of glucuronic acid conjugates is a suitable method for the selective and ultra-sensitive analysis of these compounds in urine and plasma samples. This goal is achieved by applying an indirect derivatisation procedure and laser induced fluorescence detection with a homemade detection system equipped with a continuous-wave argon-ion laser. The minimum detectable amounts for the analytes, after derivatisation, are about 3 amol using the fluorescein fluorophore. In comparison with conventional induced fluorescence detection a gain in sensitivity of over four orders of magnitude is obtained.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(88)80080-3