Endocrine responses in the llama to copulation
Nine adult female llamas were used to determine the time course for secretion of luteinizing hormone (LH) and estradiol-17β (E 2) following a single copulation (average 18 min), and progesterone (P 4) during the development of the subsequent luteal phase. Heparinized blood samples were obtained thro...
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Veröffentlicht in: | Theriogenology 1990-04, Vol.33 (4), p.891-899 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Nine adult female llamas were used to determine the time course for secretion of luteinizing hormone (LH) and estradiol-17β (E
2) following a single copulation (average 18 min), and progesterone (P
4) during the development of the subsequent luteal phase. Heparinized blood samples were obtained through an indwelling jugular cannula at 15-min intervals for up to 24 h following copulation and then once daily for up to 10 d. Luteinizing hormone, assayed by radioimmunoassay (RIA) using a monoclonal antibody 518B7 against the β subunit of bovine LH, was determined at 15 min intervals for 24 h following copulation. Estradiol-17β was determined by RIA at 4-h intervals following copulation, then daily, while P
4 values were determined daily by enzyme immunoassay. A significant increase in LH concentration was observed by 15 min after the onset of copulation, with the peak of the preovulatory surge of LH occurring at 2 h; values were basal by 7 h after copulation. Estradiol-17β values, unchanged through 18 h after copulation, tended to decline at 22 h (24 h, P |
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ISSN: | 0093-691X 1879-3231 |
DOI: | 10.1016/0093-691X(90)90824-D |