Bovine in vitro fertilization with frozen-thawed semen

Bovine in vitro fertilization with frozen-thawed semen

A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed sperm was developed. IVF frequency of in vitro matured oocytes was increased by a swimup sperm separation procedure (P=0.01) or treatment of sperm with the glycosaminoglycan...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Theriogenology 1986-04, Vol.25 (4), p.591-600
Hauptverfasser: Parrish, J.J., Susko-Parrish, J.L., Leibfried-Rutledge, M.L., Critser, E.S., Eyestone, W.H., First, N.L.
Format: Artikel
Sprache:eng
Schlagworte:
bovine
CATTLE
ESPERMATOZOO
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
FECONDATION
FECUNDACION
FERTILIZATION
FREEZE THAW CYCLES
GANADO BOVINO
GROS BETAIL
heparin
in vitro
IN VITRO EXPERIMENTATION
SPERMATOZOA
SPERMATOZOIDE
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed sperm was developed. IVF frequency of in vitro matured oocytes was increased by a swimup sperm separation procedure (P=0.01) or treatment of sperm with the glycosaminoglycan heparin (P=0.0001), but the two factors did not interact (P=0.23). Heparin was the most important factor in increasing IVF frequencies. The fertilization frequency was not affected by the batch of oocytes used (P=0.38), but bull effects were present (P
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(86)90143-3