Analysis of mycophenolic acid in dried blood spots using reversed phase high performance liquid chromatography

At our laboratory a reversed phase high performance liquid chromatography assay was developed for analysis of mycophenolic acid in dried blood spot samples. The assay was validated in the range of 0.74–23.4 mg/L and proved to be accurate and precise. The developed sample pretreatment procedure was c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-11, Vol.877 (30), p.3916-3919
Hauptverfasser:	Wilhelm, A.J., den Burger, J.C.G., Chahbouni, A., Vos, R.M., Sinjewel, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Dried blood spots Drug Stability Fingerprick Fundamental and applied biological sciences. Psychology General pharmacology Hematocrit Medical sciences Mycophenolic acid Mycophenolic Acid - blood Pharmacology. Drug treatments
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	At our laboratory a reversed phase high performance liquid chromatography assay was developed for analysis of mycophenolic acid in dried blood spot samples. The assay was validated in the range of 0.74–23.4 mg/L and proved to be accurate and precise. The developed sample pretreatment procedure was consistent and has a recovery of 95.2% for mycophenolic acid. Hematocrit showed to have influence on the physics of the blood spots and thus on the concentration of mycophenolic acid, therefore standard and control samples should be made with a standardized hematocrit.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2009.09.037