The structural analysis of the O‐glycans of the jacalin‐bound rabbit immunoglobulin G
Rabbit immunoglobulin G (IgG) is a sialoglycoprotein (2.4% carbohydrate), containing both N‐glycolyl (Neu5Gc) and N‐acetyl (Neu5Ac) neuraminic acids in a ratio of 87:13. A small fraction of rabbit IgG (about 25% of total IgG) bound to jacalin as demonstrated by affinity chromatography. The jacalin‐b...
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| Veröffentlicht in: | Biochemistry and molecular biology international 1997-07, Vol.42 (4), p.769-778 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | Rabbit immunoglobulin G (IgG) is a sialoglycoprotein (2.4% carbohydrate), containing both N‐glycolyl (Neu5Gc) and N‐acetyl (Neu5Ac) neuraminic acids in a ratio of 87:13. A small fraction of rabbit IgG (about 25% of total IgG) bound to jacalin as demonstrated by affinity chromatography. The jacalin‐bound rabbit IgG contained O‐linked glycans which were liberated from the protein by β‐elimination and isolated. Two O‐glycan structures were determined by 1H‐NMR spectroscopy, as being Neu5Gc(α2‐3)‐Gal (β31‐3)‐GalNAc‐ol and Neu5Ac(α2‐3)‐Gal(β1‐3)‐GalNAc‐ol in a ratio of 83…n17. |
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| ISSN: | 1521-6543 1039-9712 1521-6551 |
| DOI: | 10.1080/15216549700203201 |