THE EFFECT OF EXTRINSIC INCUBATION TEMPERATURE ON DEVELOPMENT OF DENGUE SEROTYPE 2 AND 4 VIRUSES IN AEDES AEGYPTI (L.)

Dengue 2 and 4 viruses obtained from dengue-infected patients were maintained in a C6/36 Aedes albopictus Skuse cell line and used to infect adult female Aedes aegypti mosquitoes. Each serotype was mixed separately with fresh human erythrocytes and fed to adult female mosquitoes using an artificial...

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Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 2009-09, Vol.40 (5), p.942-950
Hauptverfasser:	ROHANI, A, WONG, Y. C, ZAMRE, I, LEE, H. L, ZURAINEE, M. N
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - virology Animals Arboviroses Biological and medical sciences Dengue fevers Dengue Virus - growth & development Female General aspects Hot Temperature Human viral diseases Humans Infectious diseases Insect Vectors - virology Medical sciences Reverse Transcriptase Polymerase Chain Reaction Serotyping Tropical viral diseases Viral diseases
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Zusammenfassung:	Dengue 2 and 4 viruses obtained from dengue-infected patients were maintained in a C6/36 Aedes albopictus Skuse cell line and used to infect adult female Aedes aegypti mosquitoes. Each serotype was mixed separately with fresh human erythrocytes and fed to adult female mosquitoes using an artificial membrane feeding technique. Fully engorged mosquitoes were selected and retained at 26 degrees C, 28 degrees C and 30 degrees C to observe dengue virus development in Aedes vectors. Virus detection was carried out by reverse-transcriptase polymerase chain reaction (RT-PCR). The virus was first detected on Day 9 at 26 degrees C and 28 degrees C and on Day 5 at 30 degrees C for both dengue 2 and 4. The study shows the incubation period of the viruses decreased when the extrinsic incubation temperature increases.
ISSN:	0125-1562