Multi-locus variable number tandem repeat analysis for Escherichia coli causing extraintestinal infections

Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible a...

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Veröffentlicht in:	Journal of microbiological methods 2009-11, Vol.79 (2), p.211-213
Hauptverfasser:	Manges, Amee R., Tellis, Patricia A., Vincent, Caroline, Lifeso, Kimberley, Geneau, Geneviève, Reid-Smith, Richard J., Boerlin, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Typing Techniques - methods Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cluster Analysis DNA Fingerprinting - methods DNA, Bacterial - genetics Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Fundamental and applied biological sciences. Psychology Genotype Humans Meat - microbiology Microbiology Minisatellite Repeats Molecular epidemiology Molecular Epidemiology - methods Multi-locus variable number tandem repeat analysis (MLVA) Reproducibility of Results Sensitivity and Specificity Variable number tandem repeat (VNTR)
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Beschreibung
Zusammenfassung:	Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2009.09.006