The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA

The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA

Abstract To improve the transfection efficiency of polycations with DNA, we synthesized poly(ester–co–urethane)(PEU-g-PEI800) with short chain PEI800 in the side chain, and poly(ester–co–urethane)(PEU) without short chain PEI800. Both PEU-g-PEI800 and PEU, readily self-assembled with plasmid DNA (pC...

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Veröffentlicht in:Biomaterials 2009-12, Vol.30 (34), p.6665-6673
Hauptverfasser: Liu, Xin-Yi, Ho, Wen-Yueh, Hung, Wei Jing, Shau, Min-Da
Format: Artikel
Sprache:eng
Schlagworte:
Advanced Basic Science
Buffering capacity
Cations
Dentistry
DNA - administration & dosage
DNA - chemistry
DNA - genetics
Hydrolysis
Light
Magnetic Resonance Spectroscopy
Microscopy, Atomic Force
Plasmids
poly(ester–co–urethane)
Poly(ethylenimine)
Polyesters - chemistry
Polyethyleneimine - chemistry
Polyurethanes - chemistry
Scattering, Radiation
Transfection
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Zusammenfassung:Abstract To improve the transfection efficiency of polycations with DNA, we synthesized poly(ester–co–urethane)(PEU-g-PEI800) with short chain PEI800 in the side chain, and poly(ester–co–urethane)(PEU) without short chain PEI800. Both PEU-g-PEI800 and PEU, readily self-assembled with plasmid DNA (pCMV-βgal) in a HEPES buffer, were characterized by dynamic light scattering and zeta-potential. The results reveal that PEU-g-PEI800 and PEU were able to self-assemble particles with DNA and yield nano-sized complexes (
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2009.08.052