Escherichia coli shares T- and B-lymphocyte epitopes with Schistosoma mansoni

In this study, we tested the cross-reaction between crude Escherichia coli antigen (ECA) and 3 crude Schistosoma mansoni antigens. The schistosomal antigens used were cercarial antigen preparation (CAP), soluble worm antigen preparation (SWAP), and soluble egg antigen (SEA). Four groups each of 3 mi...

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Veröffentlicht in:Journal of infection in developing countries 2009-04, Vol.3 (3), p.206-217
Hauptverfasser: Bahgat, Mahmoud Mohamed, Gaber, Hanaa Mohamed, Maghraby, Amany Sayed
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Sprache:eng
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Zusammenfassung:In this study, we tested the cross-reaction between crude Escherichia coli antigen (ECA) and 3 crude Schistosoma mansoni antigens. The schistosomal antigens used were cercarial antigen preparation (CAP), soluble worm antigen preparation (SWAP), and soluble egg antigen (SEA). Four groups each of 3 mice received 2 intraperotineal immunizations with the above-mentioned antigens at a two-week interval. The dose of the ECA was 20 microg/100 microl PBS/mouse and that of any of the used schistosomal antigens was 50 microg/100 microl PBS/mouse. IgM and IgG reactivities and cross-reactivities were tested in individual immunized mice sera (IMS) against the above-mentioned antigens by ELISA and Western blotting. The changes in the B, CD4+ and CD8+ -T cells' counts post immunization were recorded. Priming with ECA caused significant increases in IgM (P
ISSN:2036-6590
1972-2680
DOI:10.3855/jidc.37