Escherichia coli shares T- and B-lymphocyte epitopes with Schistosoma mansoni
In this study, we tested the cross-reaction between crude Escherichia coli antigen (ECA) and 3 crude Schistosoma mansoni antigens. The schistosomal antigens used were cercarial antigen preparation (CAP), soluble worm antigen preparation (SWAP), and soluble egg antigen (SEA). Four groups each of 3 mi...
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Veröffentlicht in: | Journal of infection in developing countries 2009-04, Vol.3 (3), p.206-217 |
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Sprache: | eng |
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Zusammenfassung: | In this study, we tested the cross-reaction between crude Escherichia coli antigen (ECA) and 3 crude Schistosoma mansoni antigens.
The schistosomal antigens used were cercarial antigen preparation (CAP), soluble worm antigen preparation (SWAP), and soluble egg antigen (SEA). Four groups each of 3 mice received 2 intraperotineal immunizations with the above-mentioned antigens at a two-week interval. The dose of the ECA was 20 microg/100 microl PBS/mouse and that of any of the used schistosomal antigens was 50 microg/100 microl PBS/mouse. IgM and IgG reactivities and cross-reactivities were tested in individual immunized mice sera (IMS) against the above-mentioned antigens by ELISA and Western blotting. The changes in the B, CD4+ and CD8+ -T cells' counts post immunization were recorded.
Priming with ECA caused significant increases in IgM (P |
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ISSN: | 2036-6590 1972-2680 |
DOI: | 10.3855/jidc.37 |