Time-efficient and convenient synthesis of [ 18F]altanserin for human PET imaging by a new work-up procedure

[ 18F]Altanserin, an important PET radioligand for the in vivo imaging of the 5-HT 2A receptor, was synthesized from its precursor nitro-altanserin in DMF or DMSO at high temperatures of 150 °C in an overall radiochemical yield (EOB) of 23–25% after 75 min. A new solid phase work-up procedure involv...

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Veröffentlicht in:Applied radiation and isotopes 2009-11, Vol.67 (11), p.2040-2043
Hauptverfasser: Massarweh, G., Kovacevic, M., Rosa-Neto, P., Evans, A.C., Diksic, M., Schirrmacher, R.
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Sprache:eng
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Zusammenfassung:[ 18F]Altanserin, an important PET radioligand for the in vivo imaging of the 5-HT 2A receptor, was synthesized from its precursor nitro-altanserin in DMF or DMSO at high temperatures of 150 °C in an overall radiochemical yield (EOB) of 23–25% after 75 min. A new solid phase work-up procedure involving the acidification of the crude reaction mixture and a C18-SepPak-solid phase separation preceded the final HPLC purification. This led to a significantly reduced synthesis time as a result of a stable and early elution from the HPLC column using improved HPLC conditions (MeOH/THF/NaOAc 0.05 N pH 5: 27/18/55, flow: 5 mL/min, Symetry Prep 7 μm C18 (Waters)). The synthesis was performed semi-automatically in a modified GE TracerLab synthesis module using an in-house-developed program. The synthesized [ 18F]altanserin was used in our ongoing human and animal PET imaging studies.
ISSN:0969-8043
1872-9800
DOI:10.1016/j.apradiso.2009.07.020