Bordetella bronchiseptica aroA mutant as a live vaccine vehicle for heterologous porcine circovirus type 2 major capsid protein expression

Porcine circovirus type 2 (PCV2) infections cause important respiratory diseases in the pig industry and are associated with many bacterial, mycoplasmal, and viral complications. In this study, a heterologous PCV2 major capsid protein (MCP) was expressed in the Bordetella bronchiseptica aroA mutant...

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Veröffentlicht in:	Veterinary microbiology 2009-09, Vol.138 (3), p.318-324
Hauptverfasser:	Kim, Taejung, Toan, Nguyen Tat, Seo, Jayoung, Jung, Bockgie, Lee, Jaeil, Lee, Bongjoo
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Intranasal Animals antibody formation Applied microbiology Bacteriology Biological and medical sciences Bordetella bronchiseptica Bordetella bronchiseptica - genetics Capsid Proteins - immunology Circovirus - immunology Cloning, Molecular coat proteins disease prevention Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Bacterial Heterologous antigen expression vehicle immune response intranasal administration live vaccines lymph nodes Mice Microbiology Miscellaneous mutants Mutation Porcine circovirus Porcine circovirus type 2 Porcine Postweaning Multisystemic Wasting Syndrome - prevention & control Porcine Postweaning Multisystemic Wasting Syndrome - virology postweaning multisystemic wasting syndrome protective effect recombinant vaccines Swine swine diseases vaccination vaccine development Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) vector vaccines viral antigens Viral Proteins - genetics Viral Proteins - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology Virology
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Zusammenfassung:	Porcine circovirus type 2 (PCV2) infections cause important respiratory diseases in the pig industry and are associated with many bacterial, mycoplasmal, and viral complications. In this study, a heterologous PCV2 major capsid protein (MCP) was expressed in the Bordetella bronchiseptica aroA mutant strain (BBS-MCP) and used as a live vaccine vehicle. Mice and pigs were immunized with live BBS-MCP via the intranasal route. The antibodies against MCP were induced successfully in the serum as determined by ELISA. In the PCV2 challenge experiment, viral DNA was removed successfully from the lymph nodes of pigs vaccinated with live BBS-MCP. Overall, BBS-MCP is believed to be a good candidate for the development of a live-attenuated vaccine against PCV2 infections.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2009.04.014