The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function
Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) car...
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| Veröffentlicht in: | Toxicology in vitro 1998-12, Vol.12 (6), p.649-659 |
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| container_title | Toxicology in vitro |
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| creator | Stone, V. Shaw, J. Brown, D.M. MacNee, W. Faux, S.P. Donaldson, K. |
| description | Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) carbon black (CB) particles in comparison with quartz on A549 human type II alveolar epithelial cells, particularly with respect to the oxidative properties of these particles. Treatment with fine CB (diameter 260
nm), and quartz (up to 0.78
μg/mm
2) for 24 hours significantly (
P |
| doi_str_mv | 10.1016/S0887-2333(98)00050-2 |
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nm), and quartz (up to 0.78
μg/mm
2) for 24 hours significantly (
P<0.05) decreased the A549 cells metabolic competence, as measured by the ability to reduce MTT to a formazan product. The inhibitory effects of uf CB only became significantly different (
P<0.05) relative to the control at 48 hours, by which time the effects of fine CB and quartz were no longer significant. The inhibition of MTT reduction by uf CB was prevented by the hydroxyl radical scavenger mannitol (2
m
m). In addition, measurement of reactive oxygen species production using supercoiled plasmid DNA showed that uf CB exhibited significantly more free radical activity than fine CB (
P<0.05). In the absence of serum, uf CB depleted reduced glutathione at 6 hours (
P<0.008). In contrast, CB did not significantly alter reduced or oxidized glutathione. Hence, compared with fine CB, uf CB exhibited greater free radical activity, greater inhibition of the reduction of MTT at 48 hours (prevented by mannitol) and a depletion of reduced glutathione. These results suggest that uf CB induces a greater oxidative stress than fine CB, and that this may play a role in the toxicological effects of this ultrafine particle.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/S0887-2333(98)00050-2</identifier><identifier>PMID: 20654455</identifier><identifier>CODEN: TIVIEQ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Air ; Biological and medical sciences ; carbon black ; Environmental pollutants toxicology ; epithelial cells ; glutathione ; lung ; Medical sciences ; oxidative stress ; quartz ; Toxicology ; ultrafine particles</subject><ispartof>Toxicology in vitro, 1998-12, Vol.12 (6), p.649-659</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-db5bab95acc8fa33139327ac5f611a3d0f7182444c7a987fe5b0b8e20add9ca23</citedby><cites>FETCH-LOGICAL-c488t-db5bab95acc8fa33139327ac5f611a3d0f7182444c7a987fe5b0b8e20add9ca23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0887-2333(98)00050-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1638896$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20654455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stone, V.</creatorcontrib><creatorcontrib>Shaw, J.</creatorcontrib><creatorcontrib>Brown, D.M.</creatorcontrib><creatorcontrib>MacNee, W.</creatorcontrib><creatorcontrib>Faux, S.P.</creatorcontrib><creatorcontrib>Donaldson, K.</creatorcontrib><title>The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) carbon black (CB) particles in comparison with quartz on A549 human type II alveolar epithelial cells, particularly with respect to the oxidative properties of these particles. Treatment with fine CB (diameter 260
nm), and quartz (up to 0.78
μg/mm
2) for 24 hours significantly (
P<0.05) decreased the A549 cells metabolic competence, as measured by the ability to reduce MTT to a formazan product. The inhibitory effects of uf CB only became significantly different (
P<0.05) relative to the control at 48 hours, by which time the effects of fine CB and quartz were no longer significant. The inhibition of MTT reduction by uf CB was prevented by the hydroxyl radical scavenger mannitol (2
m
m). In addition, measurement of reactive oxygen species production using supercoiled plasmid DNA showed that uf CB exhibited significantly more free radical activity than fine CB (
P<0.05). In the absence of serum, uf CB depleted reduced glutathione at 6 hours (
P<0.008). In contrast, CB did not significantly alter reduced or oxidized glutathione. Hence, compared with fine CB, uf CB exhibited greater free radical activity, greater inhibition of the reduction of MTT at 48 hours (prevented by mannitol) and a depletion of reduced glutathione. These results suggest that uf CB induces a greater oxidative stress than fine CB, and that this may play a role in the toxicological effects of this ultrafine particle.</description><subject>Air</subject><subject>Biological and medical sciences</subject><subject>carbon black</subject><subject>Environmental pollutants toxicology</subject><subject>epithelial cells</subject><subject>glutathione</subject><subject>lung</subject><subject>Medical sciences</subject><subject>oxidative stress</subject><subject>quartz</subject><subject>Toxicology</subject><subject>ultrafine particles</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkU1vFSEUhomxsdfqT9CwMH4sRmFgBmZlTONX0sSFdU3OMAeLcocrME3772V6r-3Org45PO_h5byEPOPsLWe8f_edaa2aVgjxetBvGGMda9oHZMO1GhrBlXpINrfIMXmc868V0i17RI5b1ndSdt2GLOcXSFMMSKOj8cpPUPwl0lwS5kz9TEu931Ugzj9xqo0LP_oS0zVF59CWVbaEksD5GamFNMaZjgHsb1oPuPNVHzwEajEE6pbZFh_nJ-TIQcj49FBPyI9PH89PvzRn3z5_Pf1w1lipdWmmsRthHDqwVjsQgotBtAps53rOQUzMKa5bKaVVMGjlsBvZqLFlME2DhVackFf7ufUHfxbMxWx9Xp3AjHHJRgnJuORKVPLlf0muuNSMswp2e9CmmHNCZ3bJbyFdG87Mmoy5ScasazeDNjfJmNXK88MDy7jF6Vb1L4oKvDgAkC0El2C2Pt9N74XWQ1-x93sM694uPSaTrcfZ4uRTzcNM0d_j5C9Xoawl</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Stone, V.</creator><creator>Shaw, J.</creator><creator>Brown, D.M.</creator><creator>MacNee, W.</creator><creator>Faux, S.P.</creator><creator>Donaldson, K.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function</title><author>Stone, V. ; Shaw, J. ; Brown, D.M. ; MacNee, W. ; Faux, S.P. ; Donaldson, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-db5bab95acc8fa33139327ac5f611a3d0f7182444c7a987fe5b0b8e20add9ca23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Air</topic><topic>Biological and medical sciences</topic><topic>carbon black</topic><topic>Environmental pollutants toxicology</topic><topic>epithelial cells</topic><topic>glutathione</topic><topic>lung</topic><topic>Medical sciences</topic><topic>oxidative stress</topic><topic>quartz</topic><topic>Toxicology</topic><topic>ultrafine particles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stone, V.</creatorcontrib><creatorcontrib>Shaw, J.</creatorcontrib><creatorcontrib>Brown, D.M.</creatorcontrib><creatorcontrib>MacNee, W.</creatorcontrib><creatorcontrib>Faux, S.P.</creatorcontrib><creatorcontrib>Donaldson, K.</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stone, V.</au><au>Shaw, J.</au><au>Brown, D.M.</au><au>MacNee, W.</au><au>Faux, S.P.</au><au>Donaldson, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>12</volume><issue>6</issue><spage>649</spage><epage>659</epage><pages>649-659</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><coden>TIVIEQ</coden><abstract>Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) carbon black (CB) particles in comparison with quartz on A549 human type II alveolar epithelial cells, particularly with respect to the oxidative properties of these particles. Treatment with fine CB (diameter 260
nm), and quartz (up to 0.78
μg/mm
2) for 24 hours significantly (
P<0.05) decreased the A549 cells metabolic competence, as measured by the ability to reduce MTT to a formazan product. The inhibitory effects of uf CB only became significantly different (
P<0.05) relative to the control at 48 hours, by which time the effects of fine CB and quartz were no longer significant. The inhibition of MTT reduction by uf CB was prevented by the hydroxyl radical scavenger mannitol (2
m
m). In addition, measurement of reactive oxygen species production using supercoiled plasmid DNA showed that uf CB exhibited significantly more free radical activity than fine CB (
P<0.05). In the absence of serum, uf CB depleted reduced glutathione at 6 hours (
P<0.008). In contrast, CB did not significantly alter reduced or oxidized glutathione. Hence, compared with fine CB, uf CB exhibited greater free radical activity, greater inhibition of the reduction of MTT at 48 hours (prevented by mannitol) and a depletion of reduced glutathione. These results suggest that uf CB induces a greater oxidative stress than fine CB, and that this may play a role in the toxicological effects of this ultrafine particle.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>20654455</pmid><doi>10.1016/S0887-2333(98)00050-2</doi><tpages>11</tpages></addata></record> |
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| subjects | Air Biological and medical sciences carbon black Environmental pollutants toxicology epithelial cells glutathione lung Medical sciences oxidative stress quartz Toxicology ultrafine particles |
| title | The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function |
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