The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function

Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) carbon black (CB) particles in comparison with quartz on A549 human type II alveolar epithelial cells, particularly with respect to the oxidative properties of these particles. Treatment with fine CB (diameter 260 nm), and quartz (up to 0.78 μg/mm 2) for 24 hours significantly ( P