Presenilin-1 in Neuroblastoma Cells

Presenilin-1 expression was investigated in Neuro-2a cells using the αPS1Loop antibody. The bulk of the immunoreactivity was found as the ∼18 kDa C-terminal fragment. Hydrogen peroxide treatment of the Neuro-2a cells for 24 hours resulted in a concentration-dependent cytotoxicity with a critical concentration of hydrogen peroxide of approximately 20 μ m. Treatment of the cells for 24 hr with 10, 20 or 30 μ m hydrogen peroxide did not produce obvious effects upon presenilin-1 C-terminal fragment immunoreactivity. It is suggested that the loss of presenilin-1-mediated neuroprotection that has been proposed to occur in Alzheimer's disease is not secondary to hydrogen peroxide-mediated cell stress.