Isolation of VanA-type vancomycin-resistant Enterococcus strains from domestic poultry products with enrichment by incubation in buffered peptone water at 42 degrees C

Eight VanA-type enterococcal strains were isolated from 8 of 171 domestic poultry products by using enrichment by incubation in buffered peptone water at 35 degrees C and 42 degrees C. The pulsed-field gel electrophoresis patterns of all six VanA-type Enterococcus faecalis isolates were nearly indis...

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Veröffentlicht in:	Applied and environmental microbiology 2010-08, Vol.76 (15), p.5317-5320
Hauptverfasser:	Harada, Tetsuya, Kanki, Masashi, Kawai, Takao, Taguchi, Masumi, Asao, Tsutomu, Kumeda, Yuko
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Typing Techniques Bacteriological Techniques - methods Carbon-Oxygen Ligases - genetics Cluster Analysis Culture Media - chemistry DNA Fingerprinting Electrophoresis, Gel, Pulsed-Field Enterococcus - classification Enterococcus - drug effects Enterococcus - genetics Enterococcus - isolation & purification Japan Peptones Poultry Products - microbiology Vancomycin Resistance Water
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Zusammenfassung:	Eight VanA-type enterococcal strains were isolated from 8 of 171 domestic poultry products by using enrichment by incubation in buffered peptone water at 35 degrees C and 42 degrees C. The pulsed-field gel electrophoresis patterns of all six VanA-type Enterococcus faecalis isolates were nearly indistinguishable, indicating the presence of a specific clone in Japan.
ISSN:	1098-5336
DOI:	10.1128/AEM.00071-10