Kinase-Actuated Immunoaggregation of Peptide-Conjugated Gold Nanoparticles

Kinase-Actuated Immunoaggregation of Peptide-Conjugated Gold Nanoparticles

A simple and sensitive one‐step approach for the rapid colorimetric detection of v‐Src kinase using functionalized gold nanoparticles (NPs) in solution is reported. The generic concept operates without labeled ATP‐derivatives, and signals are recorded directly by spectrophotometry. For the first tim...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2010-07, Vol.6 (14), p.1509-1513
Hauptverfasser: Gupta, Shalini, Andresen, Heiko, Ghadiali, James E., Stevens, Molly M.
Format: Artikel
Sprache:eng
Schlagworte:
aggregation
Gold - chemistry
gold nanoparticles
kinase
Metal Nanoparticles
Peptides - chemistry
phosphorylation
Phosphotransferases - chemistry
surface plasmon resonance
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Beschreibung
Zusammenfassung:A simple and sensitive one‐step approach for the rapid colorimetric detection of v‐Src kinase using functionalized gold nanoparticles (NPs) in solution is reported. The generic concept operates without labeled ATP‐derivatives, and signals are recorded directly by spectrophotometry. For the first time, surface plasmon resonance spectroscopy is applied to an NP system and compared to a semi‐quantitative NP reaction model that was developed to determine the association rate constants and the rate‐limiting step of the NP agglutination process.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201000099