Identification of NbME MITE families: Potential molecular markers in the microsporidia Nosema bombycis

Six novel families of miniature inverted-repeat transposable elements (MITEs) were characterized in the microsporidia Nosema bombycis and were named NbMEs. The structural characteristics and the distribution of NbME copies in the N. bombycis genome were investigated, and it was found that portions o...

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Veröffentlicht in:	Journal of invertebrate pathology 2010, Vol.103 (1), p.48-52
Hauptverfasser:	Xu, Jinshan, Wang, Min, Zhang, Xiaoyan, Tang, Fahui, Pan, Guoqing, Zhou, Zeyang
Format:	Artikel
Sprache:	eng
Schlagworte:	amplified fragment length polymorphism Animal productions Animals Base Sequence Biological and medical sciences consensus sequence DNA Transposable Elements - genetics DNA, Fungal - genetics entomopathogenic fungi Fundamental and applied biological sciences. Psychology Fungi genetic markers genetic polymorphism genetic variation Genome, Fungal - genetics Insecta Invertebrates Inverted Repeat Sequences - genetics miniature inverted-repeat transposable elements (MITES) MITEs Molecular markers Molecular Sequence Data Nosema - genetics Nosema bombycis pathogen identification Plant cytology, morphology, systematics, chorology and evolution Polymorphisms sequence analysis Sericulture strain differences Terrestrial animal productions Thallophyta Transposon transposons
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Zusammenfassung:	Six novel families of miniature inverted-repeat transposable elements (MITEs) were characterized in the microsporidia Nosema bombycis and were named NbMEs. The structural characteristics and the distribution of NbME copies in the N. bombycis genome were investigated, and it was found that portions of NbMEs are associated with gene sections. Potential molecular markers for various N. bombycis strains were identified in this study through utilization of the MITE-AFLP technique. Three distinct pathogenic isolates collected from different areas were distinguished, and polymorphisms were detected using the NbME5 marker, thereby establishing this NbME as a potential marker for studying isolate variation in N. bombycis.
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2009.10.011