Effects of chitosan-coated pressed calcium sulfate pellets combined with recombinant human bone morphogenetic protein 2 on bone formation in femoral condyle-contained bone defects

Calcium sulfate has a rapid degradation rate and little osteoinductive capability. Chitosan-coated pressed calcium sulfate pellets combined with recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed that exhibit decreased resorption speed and increased compressive strength and osteoinduction. A rabbit femoral condyle-contained bone defect model was used to study the restoration of the defects treated with chitosan-coated pressed calcium sulfate pellets combined with rhBMP-2, chitosan-coated pressed calcium sulfate pellets, and uncoated pressed calcium sulfate pellets. No pellets were implanted in the control group. After 3 and 13 weeks, the results indicated that chitosan-coated pressed calcium sulfate pellets exhibited relatively slower resorption that closely coincides with the growth rate of new bone and enhanced osteogenesis when combined with rhBMP-2.