Assessment of the Reproducibility of Random Hexapeptide Peptide Library-Based Protein Normalization

The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commerc...

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Veröffentlicht in:	Journal of proteome research 2010-02, Vol.9 (2), p.1144-1149
Hauptverfasser:	Dwivedi, Ravi C, Krokhin, Oleg V, Cortens, John P, Wilkins, John A
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Oligopeptides - chemistry Peptide Library Proteins - chemistry Proteome Reproducibility of Results Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry
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Zusammenfassung:	The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr900608z