Hot topic: Enhancing omega-3 fatty acids in milk fat of dairy cows by using stearidonic acid-enriched soybean oil from genetically modified soybeans

Very long chain n-3 fatty acids such as eicosapentaenoic acid (EPA; 20:5n-3) are important in human cardiac health and the prevention of chronic diseases, but food sources are limited. Stearidonic acid (SDA; 18:4n-3) is an n-3 fatty acid that humans are able to convert to EPA. In utilizing SDA-enhanced soybean oil (SBO) derived from genetically modified soybeans, our objectives were to examine the potential to increase the n-3 fatty acid content of milk fat and to determine the efficiency of SDA uptake from the digestive tract and transfer to milk fat. Three multiparous, rumen-fistulated Holstein cows were assigned randomly in a 3 × 3 Latin square design to the following treatments: 1) control (no oil infusion); 2) abomasal infusion of SDA-enhanced SBO (SDA-abo); and 3) ruminal infusion of SDA-enhanced SBO (SDA-rum). The SDA-enhanced SBO contained 27.1% SDA, 10.4% α-linolenic acid, and 7.2% γ-linolenic acid. Oil infusions provided 57 g/d of SDA with equal amounts of oil infused into either the rumen or abomasum at 6-h intervals over a 7-d infusion period. Cow numbers were limited and no treatment differences were detected for DMI or milk production (22.9±0.5 kg/d and 32.3±0.9 kg/d, respectively; least squares means ± SE), milk protein percentage and yield (3.24±0.04% and 1.03±0.02 kg/d), or lactose percentage and yield (4.88±0.05% and 1.55±0.05 kg/d). Treatment also had no effect on milk fat yield (1.36±0.03 kg/d), but milk fat percentage was lower for the SDA-rum treatment (4.04±0.04% vs. 4.30±0.04% for control and 4.41±0.05% for SDA-abo). The SDA-abo treatment increased n-3 fatty acids to 3.9% of total milk fatty acids, a value more than 5-fold greater than that for the control. Expressed as a percentage of total milk fatty acids, values (least squares means ± SE) for the SDA-abo treatment were 1.55±0.03% for α-linolenic acid (18:3n-3), 1.86±0.02 for SDA, 0.23 ±