Validation of reducing-difference procedure for the interpretation of non-polarized infrared spectra of n-component solid mixtures

Validation of reducing-difference procedure for the interpretation of non-polarized infrared spectra of n-component solid mixtures

Validation of reducing-difference procedure based on subtracting of non-polarized infrared (IR) spectra of n-component solid mixtures is presented. The accuracy and precision are established. The limits of detection are 3.0, 2.5, 1.5 and 1.0 wt.% for 5-, 4-, 3-and 2-component mixtures, respectively....

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Veröffentlicht in:Talanta (Oxford) 2006-06, Vol.69 (4), p.822-828
Hauptverfasser: Ivanova, Bojidarka B., Tsalev, Dimiter L., Arnaudov, Michail G.
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Analytical chemistry
Chemistry
Exact sciences and technology
Fourier transform infrared spectroscopy (FT-IR)
Limit of detections
n-Component systems
Peptides
Reducing-difference procedure
Smoothing methods
Spectrometric and optical methods
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Zusammenfassung:Validation of reducing-difference procedure based on subtracting of non-polarized infrared (IR) spectra of n-component solid mixtures is presented. The accuracy and precision are established. The limits of detection are 3.0, 2.5, 1.5 and 1.0 wt.% for 5-, 4-, 3-and 2-component mixtures, respectively. Smoothing procedures for IR spectral analysis, based on Savitzky–Golay or Fourier methods are applied as well. The mean values and relative standard deviations for peak position ( ν i ) and integral absorbance ( A i ) obtained by this data processing approach have been examined using Student's t-test.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2005.11.026