Determination of proline in wine using flow injection analysis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection

Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision o...

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Veröffentlicht in:Talanta (Oxford) 2004-11, Vol.64 (4), p.894-898
Hauptverfasser: Costin, Jason W., Barnett, Neil W., Lewis, Simon W.
Format: Artikel
Sprache:eng
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Zusammenfassung:Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10 −6 M) and the detection limit was 1 × 10 −8 M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy) 3 3+ consumption and its associated effect on accuracy.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2004.03.065