Simultaneous spectrofluorimetric determination of the rare earths with calcein

The reactivity of the fluorescent reagent calcein with the trivalent cations of the rare earths has been spectrofluorimetrically studied in aqueous solution. Optimum excitation and emission wavelengths were 492–497 and 519–522 nm, respectively. Optimum pH was in the range 6.0–9.2. The stoichiometry of the complexes was 1:1. A direct, rapid and sensitive method for the determination of rare earth mixtures has been proposed with a detection limit of 4.49×10−8 M and a coefficient of variation of 0.82%.