Bovine serum albumin as a means to immobilize DNA on a silver-plated bulk acoustic wave DNA biosensor

A simple, inexpensive, on-line bulk acoustic wave (BAW) DNA biosensor is proposed by using an Ag-plated surface rather than the conventional Au surface. Bovine serum albumin (BSA) is used as an active coating for DNA immobilization. Impedance analysis reveals that the film composed of didodecyl dith...

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Veröffentlicht in:	Talanta (Oxford) 1998-05, Vol.46 (1), p.171-178
Hauptverfasser:	Zhang, Hong, Wang, Ronghui, Tan, Huwei, Nie, Lihua, Yao, Shouzhuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensors Biotechnology Bovine serum albumin Bulk acoustic wave DNA biosensor Fundamental and applied biological sciences. Psychology Immobilization Methods. Procedures. Technologies Silver surface Various methods and equipments
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Zusammenfassung:	A simple, inexpensive, on-line bulk acoustic wave (BAW) DNA biosensor is proposed by using an Ag-plated surface rather than the conventional Au surface. Bovine serum albumin (BSA) is used as an active coating for DNA immobilization. Impedance analysis reveals that the film composed of didodecyl dithiono-oxamide (DDDTO) and BSA can dramatically enhance the amount of immobilized DNA. Detection of dissociation and hybridization of immobilized DNA is demonstrated as an example of the potential application of this type of acoustic wave DNA biosensor in clinical practice.
ISSN:	0039-9140 1873-3573
DOI:	10.1016/S0039-9140(97)00271-3