Simultaneous determination of uric acid and creatinine in urine by an eco-friendly solvent-free high performance liquid chromatographic method
A simple, rapid, and eco-friendly analytical method for simultaneous determination of uric acid and creatinine in urine applying high performance liquid chromatography (HPLC) is described. After dilution, de-protein, and filtration, the sample solution was injected to separate the species with C-18...
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| Veröffentlicht in: | Talanta (Oxford) 2002-10, Vol.58 (4), p.711-717 |
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| creator | Jen, Jen-Fon Hsiao, Shih-Liang Liu, Kang-Hsiung |
| description | A simple, rapid, and eco-friendly analytical method for simultaneous determination of uric acid and creatinine in urine applying high performance liquid chromatography (HPLC) is described. After dilution, de-protein, and filtration, the sample solution was injected to separate the species with C-18 column by an eluent containing 0.05 M ammonium phosphate buffer at pH 7.4. An UV detector was used to monitor the separation of species at 235 nm. Optimum conditions for separation and detection were investigated. Results indicated that under optimized condition measurements were achieved within 13 min. The detection limits were 0.127 and 0.156 μg ml−1 for uric acid and creatinine respectively. The recovery was 95% (0.57% RSD) for uric acid and 99.2% (0.98% RSD) for creatinine, from five measurements. Real urine specimens were tested. |
| doi_str_mv | 10.1016/S0039-9140(02)00377-6 |
| format | Article |
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After dilution, de-protein, and filtration, the sample solution was injected to separate the species with C-18 column by an eluent containing 0.05 M ammonium phosphate buffer at pH 7.4. An UV detector was used to monitor the separation of species at 235 nm. Optimum conditions for separation and detection were investigated. Results indicated that under optimized condition measurements were achieved within 13 min. The detection limits were 0.127 and 0.156 μg ml−1 for uric acid and creatinine respectively. The recovery was 95% (0.57% RSD) for uric acid and 99.2% (0.98% RSD) for creatinine, from five measurements. Real urine specimens were tested.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/S0039-9140(02)00377-6</identifier><identifier>PMID: 18968800</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Creatinine ; Environmental-friendly ; Fundamental and applied biological sciences. Psychology ; High performance liquid chromatography ; Intermediary metabolites. 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Psychology</subject><subject>High performance liquid chromatography</subject><subject>Intermediary metabolites. Miscellaneous</subject><subject>Other biological molecules</subject><subject>Uric acid</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EotvCI4B8QcAhZRznj31CqKKAVIlDy9ly7EljlNhb26m0L9Fnxttd0WNPtuf7zYz1fYS8Y3DOgHVfrgG4rCRr4BPUn8uj76vuBdkw0fOKtz1_STb_kRNymtJfAKg58NfkhAnZCQGwIQ_XblnnrD2GNVGLGePivM4ueBpGukZnqDbOUu0tNRGL4p1H6vxeK5dhVySKJlRjdOjtvKMpzPfocykg0sndTnSLcQxx0d4gnd3dWuaZKYZF53Ab9XYqSxbMU7BvyKtRzwnfHs8z8ufy-83Fz-rq949fF9-uKtO0IlcNB4Maa2k6bbmQA2uGoRUDyAEF9IZZ0zApa9QdYwJY27S2HXgnG8bH0Q78jHw8zN3GcLdiympxyeA8H4xQPeeiFm3bFbI9kCaGlCKOahvdouNOMVD7JNRjEmpvs4JaPSah9n3vjxvWYUH71HW0vgAfjoBORs9jLO649MRxyaHveOG-Hjgsftw7jCqZYrRB6yKarGxwz3zlH4HqqD4</recordid><startdate>20021016</startdate><enddate>20021016</enddate><creator>Jen, Jen-Fon</creator><creator>Hsiao, Shih-Liang</creator><creator>Liu, Kang-Hsiung</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021016</creationdate><title>Simultaneous determination of uric acid and creatinine in urine by an eco-friendly solvent-free high performance liquid chromatographic method</title><author>Jen, Jen-Fon ; Hsiao, Shih-Liang ; Liu, Kang-Hsiung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-430ceae29c6ad389b14bb58b09be807c1dc41992ea611801545d5b369413ffdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Creatinine</topic><topic>Environmental-friendly</topic><topic>Fundamental and applied biological sciences. 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Miscellaneous</topic><topic>Other biological molecules</topic><topic>Uric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jen, Jen-Fon</creatorcontrib><creatorcontrib>Hsiao, Shih-Liang</creatorcontrib><creatorcontrib>Liu, Kang-Hsiung</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jen, Jen-Fon</au><au>Hsiao, Shih-Liang</au><au>Liu, Kang-Hsiung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of uric acid and creatinine in urine by an eco-friendly solvent-free high performance liquid chromatographic method</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2002-10-16</date><risdate>2002</risdate><volume>58</volume><issue>4</issue><spage>711</spage><epage>717</epage><pages>711-717</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>A simple, rapid, and eco-friendly analytical method for simultaneous determination of uric acid and creatinine in urine applying high performance liquid chromatography (HPLC) is described. 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| identifier | ISSN: 0039-9140 |
| ispartof | Talanta (Oxford), 2002-10, Vol.58 (4), p.711-717 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Creatinine Environmental-friendly Fundamental and applied biological sciences. Psychology High performance liquid chromatography Intermediary metabolites. Miscellaneous Other biological molecules Uric acid |
| title | Simultaneous determination of uric acid and creatinine in urine by an eco-friendly solvent-free high performance liquid chromatographic method |
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