Flow-injection determination of l-tyrosine in serum with immobilized tyrosinase
A flow-injection method for the determination of the serum l-tyrosine is described. The method involves the conversion of tyrosine into dopaquinone by reaction of tyrosinase, followed by derivation of the dopaquinone with fluorogenic agent 1,2-diphenylethylenediamine. Serum was deproteinized with tu...
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Veröffentlicht in: | Talanta (Oxford) 1993-07, Vol.40 (7), p.995-998 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
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Zusammenfassung: | A flow-injection method for the determination of the serum
l-tyrosine is described. The method involves the conversion of tyrosine into dopaquinone by reaction of tyrosinase, followed by derivation of the dopaquinone with fluorogenic agent 1,2-diphenylethylenediamine. Serum was deproteinized with tungstic acid. Sample solution was injected into a reactor (50 × 4 mm i.d.) packed with glass beads on which tyrosinase was immobilized. The fluorescence was detected at 480 nm (excitation at 350 nm). The calibration graph was linear for 5 × 10
−7−2 × 10
−4
M
l-tyrosine; the detection limit was 2 × 10
−7
M. |
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ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/0039-9140(93)80156-L |