Flow-injection determination of l-tyrosine in serum with immobilized tyrosinase

A flow-injection method for the determination of the serum l-tyrosine is described. The method involves the conversion of tyrosine into dopaquinone by reaction of tyrosinase, followed by derivation of the dopaquinone with fluorogenic agent 1,2-diphenylethylenediamine. Serum was deproteinized with tu...

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Veröffentlicht in:Talanta (Oxford) 1993-07, Vol.40 (7), p.995-998
Hauptverfasser: Kiba, Nobutoshi, Suzuki, Hiroshi, Furusawa, Motohisa
Format: Artikel
Sprache:eng
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Zusammenfassung:A flow-injection method for the determination of the serum l-tyrosine is described. The method involves the conversion of tyrosine into dopaquinone by reaction of tyrosinase, followed by derivation of the dopaquinone with fluorogenic agent 1,2-diphenylethylenediamine. Serum was deproteinized with tungstic acid. Sample solution was injected into a reactor (50 × 4 mm i.d.) packed with glass beads on which tyrosinase was immobilized. The fluorescence was detected at 480 nm (excitation at 350 nm). The calibration graph was linear for 5 × 10 −7−2 × 10 −4 M l-tyrosine; the detection limit was 2 × 10 −7 M.
ISSN:0039-9140
1873-3573
DOI:10.1016/0039-9140(93)80156-L