Determination of hydrogen peroxide with N,N-diethylaniline and 4-aminoantipyrine by use of an anion-exchange resin modified with manganese-tetrakis(sulphophenyl)porphine, as a substitute for peroxidase
Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H 2O 2 + N,N-diethylaniline + 4-aminoantipyrine catalyst→ quinonoid dye (λ max 550 nm) + 4H 2O....
Gespeichert in:
Veröffentlicht in: | Talanta (Oxford) 1987-07, Vol.34 (7), p.667-669 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H
2O
2 +
N,N-diethylaniline + 4-aminoantipyrine
catalyst→ quinonoid dye (λ
max 550 nm) + 4H
2O. The apparent molar absorptivity for hydrogen peroxide was 1.1 × 10
4 1.mole
−1.cm
−1, coefficient of variation 0.7%. This value is approximately 84% of that obtained by the use of peroxidase as catalyst. Similar conditions to those in the enzymatic reaction were suitable for use of the modified resin as catalyst, and the results show it to be a good substitute for peroxidase in this reaction system. |
---|---|
ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/0039-9140(87)80086-3 |