Circulating endothelial cells are elevated in patients with type 1 diabetes mellitus

ObjectiveCirculating endothelial cells (CECs) have emerged as vascular damage markers and are increased in type 2 diabetic patients. Since type 1 diabetes is associated with vascular damage, we hypothesized high CEC numbers in this patient population.MethodsThirty-nine patients with type 1 diabetes...

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Veröffentlicht in:	European journal of endocrinology 2010-04, Vol.162 (4), p.711-717
Hauptverfasser:	Asicioglu, Ebru, Gogas Yavuz, Dilek, Koc, Mehmet, Ozben, Beste, Yazici, Dilek, Deyneli, Oguzhan, Akalin, Sema
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aryldialkylphosphatase - blood Biological and medical sciences Brachial Artery - diagnostic imaging Cell Count Clinical Study Diabetes Mellitus, Type 1 - blood Diabetes Mellitus, Type 1 - diagnostic imaging Diabetes Mellitus, Type 1 - pathology Diabetes. Impaired glucose tolerance Electrocardiography Endocrine pancreas. Apud cells (diseases) Endocrinopathies Endothelium, Vascular - diagnostic imaging Endothelium, Vascular - pathology Etiopathogenesis. Screening. Investigations. Target tissue resistance Female Fundamental and applied biological sciences. Psychology Glutathione - blood Humans Male Medical sciences Microscopy, Fluorescence Oxidative Stress - immunology Statistics, Nonparametric Thiobarbituric Acid Reactive Substances - metabolism Ultrasonography Vasodilation Vertebrates: endocrinology
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Zusammenfassung:	ObjectiveCirculating endothelial cells (CECs) have emerged as vascular damage markers and are increased in type 2 diabetic patients. Since type 1 diabetes is associated with vascular damage, we hypothesized high CEC numbers in this patient population.MethodsThirty-nine patients with type 1 diabetes and 39 controls were included. CECs were isolated using anti-CD146-coated Dynabeads, stained with Ulex lectin-1, and counted by fluorescence microscopy. Endothelial function was measured as flow-mediated dilation (FMD). Thiobarbituric acid reactive substances (TBARS), total glutathione levels (GSH), and paraoxonase (PON) activity levels were measured as oxidative stress markers.ResultsPatients with type 1 diabetes mellitus had higher number of CECs (7.46±5.37 vs 2.13±1.13 cells/ml, P
ISSN:	0804-4643 1479-683X
DOI:	10.1530/EJE-09-0795