The preparation of A purified immunogenic pertactin from B. pertussis

Pertactin, an outer membrane protein of Bordetella pertussis, was purified to apparent homogeneity. The purified pertactin was first extracted from the B. pertussis cells (strain 165) by heating for 1 h at 60 degrees C, followed by DEAE-Sepharose and Affi-Gel Blue chromatography. The purified pertac...

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Veröffentlicht in:	The Egyptian journal of immunology 2007, Vol.14 (2), p.11-18
Hauptverfasser:	El-Kady, E M, Abu-Shady, H M, Tohamy, H S, Wahby, A F
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Bacterial - immunology Antigens, Bacterial - immunology Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - immunology Bacterial Outer Membrane Proteins - isolation & purification Blotting, Western Bordetella pertussis - metabolism Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Molecular Weight Pertussis Vaccine - immunology Virulence Factors, Bordetella - chemistry Virulence Factors, Bordetella - immunology Virulence Factors, Bordetella - isolation & purification
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Zusammenfassung:	Pertactin, an outer membrane protein of Bordetella pertussis, was purified to apparent homogeneity. The purified pertactin was first extracted from the B. pertussis cells (strain 165) by heating for 1 h at 60 degrees C, followed by DEAE-Sepharose and Affi-Gel Blue chromatography. The purified pertactin migrated as a single band of 69-kDa and a pl of 5.9 and retains a high immunogenicity. The rabbit anti- pertactin antisera shows high specificity in ELISA. The purified pertactin is a potential candidate as immunogen for preparation of diagnostic reagents and as a vaccine component.
ISSN:	1110-4902