Peroxidase-Like Catalytic Activity of Water-Insoluble Complex Linked Fe(III)-Thiacalix[4]arenetetrasulfonate with Tetrakis(1-methylpyridinium-4-yl)porphine via Ionic Interaction

A new water-insoluble Fe3+-TCAS[4]/TMPyP complex linked tetraanionic Fe(III)-thiacalix[4]arenetetrasulfonate (Fe3+-TCAS[4]) with tetracationic tetrakis(1-methylpyridinium-4-yl)porphine (TMPyP) via ionic interaction was prepared. The peroxidase-like catalytic activity of the Fe3+-TCAS[4]/TMPyP complex was investigated based on the dye formation reaction by oxidation of 4-aminoantipyrine and phenol with H2O2 catalyzed by peroxidase. This Fe3+-TCAS[4]/TMPyP complex showed the highest activity in pH 5.5 acetate buffer solutions, and it was applied to the photometric determination of trace amounts of H2O2. The calibration curve was linear over the range from 1.0 to 35 μg of H2O2 in a 1.0 ml sample solution. Moreover, the method using glucoseoxidase and the Fe3+-TCAS[4]/TMPyP complex was applied to the determination of glucose, and the results were satisfactory even in control sera. The Fe3+-TCAS[4]/TMPyP complex can be applied to a practical sample, such as blood or urine, as an analytical reagent for the photometric determination of H2O2 in place of peroxidase.