2-D RP/RPLC method to separate components in Fructus schisandrae chinensis

An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click β-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. β-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click β-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click β-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.