Amycolatopsis tucumanensis sp. nov., a copper-resistant actinobacterium isolated from polluted sediments

1 Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros, 4000 Tucumán, Argentina 2 Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina 3 Departamento de Microbiología y Genética, Edificio Departamental, Lab. 205, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain 4 Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina Correspondence Carlos Mauricio Abate cabate{at}proimi.org.ar A novel actinomycete strain, ABO T , isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABO T developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern ( meso -diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202 T . The DNA–DNA relatedness of strain ABO T to A. eurytherma DSM 44348 T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABO T represents a novel species in the genus Amycolatopsis , for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABO T (=DSM 45259 T =LMG 24814 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ABO T is DQ886938 . A table showing the fatty acid composition of strain ABO T , a micrograph of the aerial mycelium of A. eurytherma DSM 44348 T and an extended neighbour-joining tree based on 16S rRNA gene sequences are available as supplementary material with the online version of this paper.