Comparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome

Summary Evaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false‐positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7‐amino‐actinomycin D (7‐AAD) were compared for their...

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Veröffentlicht in:	Andrologia 2010-02, Vol.42 (1), p.20-26
Hauptverfasser:	Falzone, N., Huyser, C., Franken, D. R.
Format:	Artikel
Sprache:	eng
Schlagworte:	7-amino-actinomycin-D Acrosome - metabolism Acrosome - physiology Acrosome Reaction Adult Biological and medical sciences Dactinomycin - analogs & derivatives Dactinomycin - chemistry Dactinomycin - metabolism False Positive Reactions flow cytometry Flow Cytometry - methods Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Gynecology. Andrology. Obstetrics human spermatozoa Humans Male Male genital diseases Medical sciences Microscopy, Fluorescence Propidium - chemistry Propidium - metabolism propidium iodide Spermatozoa - metabolism Spermatozoa - physiology
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Zusammenfassung:	Summary Evaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false‐positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7‐amino‐actinomycin D (7‐AAD) were compared for their ability to stain nonviable cells post‐fixation and permeabilisation. Both the mean fluorescence and % dead cells differed significantly with time (P
ISSN:	0303-4569 1439-0272
DOI:	10.1111/j.1439-0272.2009.00949.x