LAMP Using a Disposable Pocket Warmer for Anthrax Detection, a Highly Mobile and Reliable Method for Anti-Bioterrorism

A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that u...

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Veröffentlicht in:Japanese Journal of Infectious Diseases 2010/01/29, Vol.63(1), pp.36-40
Hauptverfasser: Hatano, Ben, Maki, Takayuki, Obara, Takeyuki, Fukumoto, Hitomi, Hagisawa, Kohsuke, Matsushita, Yoshitaro, Okutani, Akiko, Bazartseren, Boldbaatar, Inoue, Satoshi, Sata, Tetsutaro, Katano, Harutaka
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Sprache:eng
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Zusammenfassung:A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that uses a disposable pocket warmer without the need for electricity (pocket-warmer LAMP). In our tests, the detection limit of the pocket-warmer LAMP was 1,000 copies of Bacillus anthracis pag and capB gene fragments per tube. The pocket-warmer LAMP also detected B. anthracis genes from DNA extracted from 0.1 volume of a B. anthracis colony. The lower detection limit of the pocket-warmer LAMP was not significantly different from that of a conventional LAMP using a heat block, and was not changed under cold (4ºC) or warm (37ºC) conditions in a Styrofoam box. The pocket-warmer LAMP could be useful against bioterrorism, and as a sensitive, reliable detection tool in areas with undependable electricity infrastructures.
ISSN:1344-6304
1884-2836
DOI:10.7883/yoken.63.36