Spontaneous Aneuploidy and Clone Formation in Adipose Tissue Stem Cells during Different Periods of Culturing

Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fl uorescent in situ hybridization. During the early passages, m...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2009-07, Vol.148 (1), p.109-112
Hauptverfasser:	Buyanovskaya, O. A, Kuleshov, N. P, Nikitina, V. A, Voronina, E. S, Katosova, L. D, Bochkov, N. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipose Tissue - cytology Aneuploidy Biomedical and Life Sciences Biomedicine Body fat Cell Biology Cell Technologies in Biology and Medicine Cells, Cultured Chromosomes, Human Clone Cells clone formation fl uorescent in situ hybridization (FISH) Humans Internal Medicine interphase nuclei Laboratory Medicine Pathology stem cells Stem Cells - cytology
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Zusammenfassung:	Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fl uorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-009-0647-3