Computational analysis and prediction for exons of PAC579 genomic sequence
To isolate the novel genes related to human hepatocellular carcinoma (HCC), we sequenced P1-derived artificial chromosome PAC579 (D17S926 locus) mapped in the minimum LOH (loss of heterozygosity) deletion region of chromosome 17p13.3 in HCC. Four novel genes mapped in this genomic sequence area were...
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Veröffentlicht in: | Science China. Life sciences 2001-10, Vol.44 (5), p.533-540 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | To isolate the novel genes related to human hepatocellular carcinoma (HCC), we sequenced P1-derived artificial chromosome PAC579 (D17S926 locus) mapped in the minimum LOH (loss of heterozygosity) deletion region of chromosome 17p13.3 in HCC. Four novel genes mapped in this genomic sequence area were isolated and cloned by wet-lab experiments, and the exons of these genes were located. 0-60 kb of this genomic sequence including the genes of interest was scanned with five different computational exon prediction programs as well as four splice site recognition programs. After analyzing and comparing the computationally predicted results with the wet-lab experiment results, some potential exons were predicted in the genomic sequence by using these programs. |
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ISSN: | 1006-9305 1674-7305 1862-2798 1869-1889 |
DOI: | 10.1007/BF02882396 |