Sequencing and bacterial expression of a novel murine alpha interferon gene

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cel...

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Veröffentlicht in:	Science China. Life sciences 1997-06, Vol.40 (3), p.277-283
1. Verfasser:	王焱 王征宇 周鸣南 蔡菊娥 孙兰英 刘新垣 B.L.Daugherty S.Pestka
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha Antiviral activity expression Genes Interferon method murine Nucleotide sequence primer-based prokaryotic sequencing
Online-Zugang:	Volltext
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