Sequencing and bacterial expression of a novel murine alpha interferon gene

Sequencing and bacterial expression of a novel murine alpha interferon gene

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cel...

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Veröffentlicht in:Science China. Life sciences 1997-06, Vol.40 (3), p.277-283
1. Verfasser: 王焱 王征宇 周鸣南 蔡菊娥 孙兰英 刘新垣 B.L.Daugherty S.Pestka
Format: Artikel
Sprache:eng
Schlagworte:
alpha
Antiviral activity
expression
Genes
Interferon
method
murine
Nucleotide sequence
primer-based
prokaryotic
sequencing
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Zusammenfassung:A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mlFN-αB has been accepted by GENEBANK.
ISSN:1674-7305
1006-9305
1869-1889
1862-2798
DOI:10.1007/BF02879088