Mapping of oxygen tension and cell distribution in a hollow-fiber bioreactor using magnetic resonance imaging

We mapped the distribution of dissolved oxygen and mammalian cells in a hollow‐fiber bioreactor (HFBR) using 19F NMR T1 relaxation time imaging measurements on an infused perfluorocarbon probe molecule and diffusion‐weighted 1H NMR imaging of water. This study shows how cell density influences dissolved oxygen concentration in the reactor and demonstrates that NMR can play an important role in defining the biochemical engineering parameters required for optimization of HFBR design and operation. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 56–61, 1997.