Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer

Objective To determine whether metabolomic profiling of spent embryo culture media correlates with reproductive potential of human embryos. Design Retrospective study. Setting Academic and a private assisted reproductive technology (ART) programs. Patient(s) Women undergoing single embryo transfer after IVF. Intervention(s) Spent embryo culture media were collected after single embryo transfer on day 3 (n = 304) or day 2 (n = 181) and analyzed by near infrared spectroscopy. Near infrared spectral regions were correlated to reproductive potential using a genetic algorithm optimization. Models of these spectral regions were used to calculate viability indices, and were validated by blinded analysis of a subset (n = 60) of samples. Implantation rates were also compared between embryos of higher (≥0.3) and lower (