Methodological Guidelines for Genetic Safety Testing of Cell Transplants

A combination of karyotyping and aneuploidy analysis by interphase fluorescent in situ hybridization is a sensitive method for evaluation of genetic stability of stem cell cultures. The methodology and specific features of preparing and analyzing the cytogenetic preparations are described as exempli...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2009-10, Vol.148 (4), p.677-683
Hauptverfasser:	Bochkov, N. P, Nikitina, V. A, Voronina, E. S, Kuleshov, N. P
Format:	Artikel
Sprache:	eng
Schlagworte:	aneuploidy Biomedical and Life Sciences Biomedicine Cell Biology Cell Culture Techniques - methods cell transplant Cells, Cultured Cytogenetics - methods Guidelines as Topic Humans In Situ Hybridization, Fluorescence - methods Internal Medicine karyotyping Karyotyping - methods Laboratory Medicine Mesenchymal Stem Cell Transplantation - adverse effects Mesenchymal Stem Cell Transplantation - methods methodological guidelines multipotent mesenchymal stromal cells Multipotent Stem Cells - cytology Multipotent Stem Cells - physiology Pathology Ploidies Stromal Cells - cytology Stromal Cells - physiology Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Beschreibung
Zusammenfassung:	A combination of karyotyping and aneuploidy analysis by interphase fluorescent in situ hybridization is a sensitive method for evaluation of genetic stability of stem cell cultures. The methodology and specific features of preparing and analyzing the cytogenetic preparations are described as exemplified by human multipotent mesenchymal stromal cells.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-010-0793-7