Purification of lactoferrin using hydroxyapatite

Lactoferrin is an important nutriceutical with various physiological functions. It is present in whey at very low concentrations. This work describes a mixed-mode (hydroxyapatite) chromatography method for one-column fractionation of lactoferrin from whey. Lactoperoxidase, a protein with similar mol...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-04, Vol.878 (13), p.976-980
Hauptverfasser:	Ng, Paul K., Yoshitake, Tomohiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Blotting, Western Chromatography, Liquid - methods Durapatite - chemistry Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology General pharmacology Hydroxyapatite Lactoferrin Lactoferrin - isolation & purification Lactoperoxidase Medical sciences Pharmacology. Drug treatments Purification Whey
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Lactoferrin is an important nutriceutical with various physiological functions. It is present in whey at very low concentrations. This work describes a mixed-mode (hydroxyapatite) chromatography method for one-column fractionation of lactoferrin from whey. Lactoperoxidase, a protein with similar molecular weight and isoelectric point, was initially desorbed from the matrix under isocratic conditions. Lactoferrin was obtained in homogeneity without lactoperoxidase activity and free from other major whey proteins such as alpha lactoalbumin and beta lactoglobulin.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2010.02.027