Single-Molecule STED Microscopy with Photostable Organic Fluorophores

The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single‐molecule level. A lateral resolution...

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Veröffentlicht in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2010-07, Vol.6 (13), p.1379-1384
Hauptverfasser:	Kasper, Robert, Harke, Benjamin, Forthmann, Carsten, Tinnefeld, Philip, Hell, Stefan W., Sauer, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Pairing - genetics Cell Line fluorescence Fluorescent Dyes - chemistry imaging Light Microscopy - methods Microscopy, Fluorescence Oligonucleotides - metabolism Organic Chemicals - chemistry Oxidation-Reduction photostability redox chemistry stimulated emission depletion microscopy
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Beschreibung
Zusammenfassung:	The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single‐molecule level. A lateral resolution of
ISSN:	1613-6810 1613-6829
DOI:	10.1002/smll.201000203