Molecular characterisation of PFGE non-typable methicillin-resistant Staphylococcus aureus in The Netherlands, 2007

In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA i...

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Veröffentlicht in:	Euro surveillance : bulletin européen sur les maladies transmissibles 2009-09, Vol.14 (38)
Hauptverfasser:	Huijsdens, X W, Bosch, T, van Santen-Verheuvel, M G, Spalburg, E, Pluister, G N, van Luit, M, Heck, M E O C, Haenen, A, de Neeling, A J
Format:	Artikel
Sprache:	eng
Schlagworte:	Electrophoresis, Gel, Pulsed-Field - methods Humans Methicillin-Resistant Staphylococcus aureus - classification Methicillin-Resistant Staphylococcus aureus - isolation & purification Molecular Probe Techniques Netherlands
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA isolates revealed 27 different spa types and two distinct SCCmec types, type IV and V. All NT-MRSA isolates were closely related based on spa and multi-locus sequence typing and belonged to the ST398 lineage. The rapid increase of NT-MRSA (ST398) isolates over the last years shows the importance of this relatively new clonal lineage.
ISSN:	1560-7917 1560-7917
DOI:	10.2807/ese.14.38.19335-en