Percentage of regulatory T cells CD4 +CD25 +CD127 − in HIV-infected patients is not reduced after cryopreservation

Interest in regulatory T cells (Treg) has been continuously growing over the last 10 years. In particular, a role for Treg has been recently reported in HIV. It is crucial to investigate their conservation over time, once sampled and purified. The objective of the present study was to investigate wh...

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Veröffentlicht in:	Journal of immunological methods 2010-05, Vol.357 (1), p.55-58
Hauptverfasser:	Venet, Fabienne, Malcus, Christophe, Ferry, Tristan, Poitevin, Françoise, Monneret, Guillaume
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences CD4 +CD25 CD4 Antigens - blood CD4 Antigens - immunology CD4 Lymphocyte Count Cohort Studies Cryopreservation Female FOXP3 Fundamental and applied biological sciences. Psychology Fundamental immunology HIV Infections - blood HIV Infections - immunology Humans Interleukin-2 Receptor alpha Subunit - blood Interleukin-2 Receptor alpha Subunit - immunology Interleukin-7 Receptor alpha Subunit - blood Interleukin-7 Receptor alpha Subunit - immunology Male Molecular immunology Regulatory T cells T-Lymphocytes, Regulatory Techniques
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Zusammenfassung:	Interest in regulatory T cells (Treg) has been continuously growing over the last 10 years. In particular, a role for Treg has been recently reported in HIV. It is crucial to investigate their conservation over time, once sampled and purified. The objective of the present study was to investigate whether cryopreservation may modify recognition and measurement of relative frequency of Treg (CD4 +CD25 +CD127 −) among PBMCs purified from 17 HIV-infected patients. Ours results indicate that cryopreservation does not affect this specific T lymphocyte subset.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/j.jim.2010.02.005