Inhibition of Na+/H+ antiport activity in sugar beet tonoplast by analogs of amiloride

The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension cultures. There is a competitive interaction between amiloride analogs and sodium. Substitution of one or bot...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology (Bethesda) 1987-09, Vol.85 (1), p.30-33
Hauptverfasser:	Blumwald, E, Cragoe, E.J. Jr, Poole, R.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines BETA VULGARIS Biological and medical sciences CATION CATIONES CATIONS Cell membranes Cell physiology CELL SUSPENSIONS Communications CULTIVO DE TEJIDOS CULTURE DE TISSUS DIURETICOS DIURETICS DIURETIQUE Fluorescence Fundamental and applied biological sciences. Psychology HIDROGENO HYDROGEN HYDROGENE Plant physiology and development Plants Plasma membrane and permeation Protons Protoplasts Rates of change SODIO SODIUM Sugar beets TISSUE CULTURE Tonoplast VACUOLA VACUOLE VACUOLES
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension cultures. There is a competitive interaction between amiloride analogs and sodium. Substitution of one or both H-atoms of the 5-amino group of amiloride (apparent Ki about 150 micromolar) resulted in a 3- to 200-fold increase in inhibitory potency of the antiport activity.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.85.1.30