Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis

Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis

The capsid protein of human immunodeficiency virus type 1 was observed to undergo proteolytic cleavage in vitro when viral lysate was incubated in the presence of dithiothreitol at acidic pH. Purified HIV-1 capsid protein was also found to be a substrate of the viral proteinase in a pH-dependent man...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2003-05, Vol.310 (1), p.16-23
Hauptverfasser: Tözsér, József, Shulenin, Sergey, Kádas, János, Boross, Péter, Bagossi, Péter, Copeland, Terry D, Nair, Bala C, Sarngadharan, Mangalasseril G, Oroszlan, Stephen
Format: Artikel
Sprache:eng
Schlagworte:
Capsid Proteins - metabolism
Cyclophilin A - pharmacology
Enzyme kinetics
HIV Integrase - metabolism
HIV Protease - metabolism
HIV-1 - metabolism
HIV-1 capsid protein
HIV-1 proteinase
Hydrogen-Ion Concentration
Integrase
Oligopeptide substrates
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Zusammenfassung:The capsid protein of human immunodeficiency virus type 1 was observed to undergo proteolytic cleavage in vitro when viral lysate was incubated in the presence of dithiothreitol at acidic pH. Purified HIV-1 capsid protein was also found to be a substrate of the viral proteinase in a pH-dependent manner; acidic pH (
ISSN:0042-6822
1096-0341
DOI:10.1016/S0042-6822(03)00074-6