Tissue distribution and subcellular localization of prephenate aminotransferase in leaves of Sorghum bicolor

The tissue and subcellular distribution of prephenate aminotransferase, an enzyme of the shikimate pathway, was investigated in protoplasts from leaves of Sorghum bicolor. Activity was detected in purified epidermal and mesophyll protoplasts, and in bundle sheath strands. After fractionation of meso...

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Veröffentlicht in:	Plant physiology (Bethesda) 1986-06, Vol.81 (2), p.711-713
Hauptverfasser:	Siehl, D.L, Singh, B.K, Conn, E.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE AMINOTRANSFERASAS AMINOTRANSFERASE AMINOTRANSFERASES BIOCHEMICAL PATHWAYS Biological and medical sciences Biosynthesis Cell biochemistry Cell physiology Chloroplasts Dehydrogenases Enzymes ENZYMIC ACTIVITY FEUILLE Fundamental and applied biological sciences. Psychology HOJAS LEAVES Mesophyll Plant physiology and development Plastids PROTOPLASM PROTOPLASMA PROTOPLASME Protoplasts Short Communications Sorghum SORGHUM BICOLOR TEJIDO (ANATOMIA) TISSU Tissue distribution TISSUES VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME
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Zusammenfassung:	The tissue and subcellular distribution of prephenate aminotransferase, an enzyme of the shikimate pathway, was investigated in protoplasts from leaves of Sorghum bicolor. Activity was detected in purified epidermal and mesophyll protoplasts, and in bundle sheath strands. After fractionation of mesophyll and epidermal protoplasts by differential centrifugation, 92% of the total prephenate aminotransferase activity was detected in the plastid fraction.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.81.2.711