Vacuolar localization of ethylene-induced chitinase in bean leaves [Phaseolus vulgaris]

The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Va...

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Veröffentlicht in:	Plant physiology (Bethesda) 1984-02, Vol.74 (2), p.442-444
Hauptverfasser:	Boller, Thomas, Urs Vögeli
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell biochemistry Cell physiology Cell walls Dextrans Enzymes Fundamental and applied biological sciences. Psychology Interphase Leaves Pathogens Plant physiology and development Plants Protoplasts Short Communications Sulfates Vacuoles
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Zusammenfassung:	The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular chitinase activity.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.74.2.442